For the primary outcome, no significant difference was observed between groups: vaccine take occurred in 62 (67%) high-dose infants versus 69 (71%) standard-dose infants (RR 0.92, 95% CI 0.67–1.24). 97 standard-dose and 92 high-dose infants completed the study per-protocol. Vaccine take was defined as detection of post-vaccination fecal vaccine shedding by real-time reverse transcription polymerase chain reaction with sequence confirmation or plasma rotavirus-specific immunoglobulin A (RV-IgA) seroconversion 4 weeks following the second dose.Ģ20 infants were enrolled and randomized (110 per group). Infants were randomized using random permuted blocks.
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Infants with congenital malformations, birth or enrollment weight <2000 gm, known immunocompromising condition, enrollment in another vaccine trial, or other household member enrolled in the study were excluded.
BIORAD CFX MANAGER USE ROX AS REFERENCES TRIAL
We performed a double-blind, parallel group, randomized controlled trial from June 2017 through June 2018 in the urban Mirpur slum of Dhaka, Bangladesh to compare vaccine take (primary outcome) among healthy infants randomized to receive either the standard dose or double the standard dose of oral Rotarix (GlaxoSmithKline) vaccine at 6 and 10 weeks of life. Increasing the inoculum of vaccine might improve vaccine response, but this approach has been inadequately explored in low-income countries. The inclusion of UNG and dUTP in PowerTrack SYBR Green Master Mix results in the degradation of any previously amplified PCR products and helps prevent contamination of subsequent qPCR reactions.Oral, live-attenuated rotavirus vaccines suffer from impaired immunogenicity and efficacy in low-income countries. For optimal results, primer concentrations should be in the range of 300–800 nM.Ĭontamination in labs that routinely run PCR is a major concern for many researchers and is the source of most false positives. It is also compatible with the Bio-Rad CFX96™, CFX384™, and iQ™5 Roche LightCycler™ 480 and Agilent Mx3005P™ systems. PowerTrack SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments. PowerTrack SYBR Green Master Mix is designed for exceptional performance in your most challenging real-time PCR applications. PowerTrack SYBR Green Master Mix contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification. The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with PowerTrack SYBR Green Master Mix.įormulated for maximum specificity and reproducibility The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes. A separate sample buffer is provided that contains an inert yellow dye.
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PowerTrack SYBR Green Master Mix contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well. Formulated with UNG/dUTP to prevent contamination of carryover PCR products.Specific and tight reproducibility in Ct’s over a broad dynamic range.Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization.Built-in visual indicators aid in reaction setup.
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Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.įeatures of PowerTrack SYBR Green Master Mix include: PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.